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How To Use The CD45 Marker

The CD45 Market is also called the leukocyte common antigen (LCA). It is a surface marker for leukocytes and also for the transmembrane protein called tyrosine phosphate. It can be expressed by tumors of a leukocytic lineage and leukocytes.

More Information About It

This monoclonal antibody has a clone called SP307. Its immunogen is the synthetic peptide from the human protein of the same name, found in the c-terminus. The isotype is the Rabbit IgG, and it has an undetermined epitope with a molecular weight between 180 and 240 kDa.

Applications

The CD45 marker can use Flow Cytometry, Western Blotting, and IHC applications.

For IHC applications, you’ll need to use a paraffin-embedded or Formalin-fixed tissue and deparaffinized slides. You’ll find a pre-diluted format, which can be used in IHC applications. Otherwise, you’ll use the concentrated version and dilute it using a ratio of 1:100. Boil the tissue section in an EDTA buffer with a pH of 8.0 to retrieve the antigen. You’ll need to do this for ten minutes and allow the concoction to cool for 20 minutes. There is an incubation period of ten minutes, as well. The positive control here is the tonsil.

For Western Blotting, you should dilute the antibody using a ratio of 1:3200, which means you cannot use the pre-diluted formula. Incubation periods are one hour at room temperature with the positive control in the Jurkat Cell Lysate.

For Flow Cytometry, you should dilute the antibody using a ratio of 1:1600, incubating for 30 minutes at a temperature of four degrees Celsius. The positive control is also the Jurkat Cell Lysate. All methods have cellular localization occurring in the membrane.

The CD45 marker can be an excellent addition to your laboratory and can help you stain and mark a variety of cells. Visit Spring Bioscience to learn more today.

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